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Amaxa amaxa basic nucleofector kit
Amaxa Basic Nucleofector Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/basic+nucleofector+kit/basic+kit+nucleofector/pmc13266002-306-14-14
Average 86 stars, based on 1 article reviews
amaxa basic nucleofector kit - by Bioz Stars, 2026-09
86/100 stars

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Transfection:

Article Title: MicroRNA-26a inhibits TGF-β-induced extracellular matrix protein expression in podocytes by targeting CTGF and is downregulated in diabetic nephropathy.
Article Snippet: .. Transfection Differentiated podocytes were transfected with miR-26a mimics or mimic negative control (NC; 30 nmol/l), miR-26a inhibitors or inhibitor NC (30 nmol/l), and siRNA for CTGF, CTDSP2 and CTDSPL (5 nmol/l) by a pulse of electroporation with Nucleofector (Amaxa Biosystems, Cologne, Germany) using the Basic Nucleofector Kit for Primary Mammalian Epithelial cells (Amaxa Biosystems) [29] (for further details, please refer to the ESM Methods). ..

Article Title: Post-stroke hippocampal neurogenesis is impaired by microvascular dysfunction and PI3K signaling in cerebral amyloid angiopathy
Article Snippet: Bands were imaged with the Odyssey CLX Imaging System (LICOR) and signal quantification was performed with Image Studio 4.0 software (LICOR). .. ReN cells were seeded in a 6-well culture plate coated with laminin and immediately transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit. ..

Article Title: Post-stroke hippocampal neurogenesis is impaired by microvascular dysfunction and PI3K signaling in cerebral amyloid angiopathy.
Article Snippet: ReN cells were seeded in a 6-well culture plate coated with laminin and immediately transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit.78 For gene silencing, cells were transfected with 0.5 mg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer SDF1 (CXCL12)-siRNAs 3 unique 27mer PI3KC2A-siRNAs, 3 unique 27mer CREB3L2-siRNAs, or trilencer-27 universal scrambled (SCR) silencer siRNA duplex as a negative control. .. ReN cells were seeded in a 6-well culture plate coated with laminin and immediately transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit.78 For gene silencing, cells were transfected with 0.5 mg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer SDF1 (CXCL12)-siRNAs 3 unique 27mer PI3KC2A-siRNAs, 3 unique 27mer CREB3L2-siRNAs, or trilencer-27 universal scrambled (SCR) silencer siRNA duplex as a negative control. ..

Article Title: Deletion of P2Y 2 receptor reveals a role for lymphotoxin-α in fatty streak formation
Article Snippet: The resultant construct was co-transfected with the pRL-TK Vector (Promega) containing Renilla luciferase reporter gene into the cultured ECs. .. A Nucleofector electroporator and a basic Nucleofector kit (Amaxa Inc., Gaithersburg, MD) were used for transfection of primary ECs according to the instructions of the manufacturer. ..

Negative Control:

Article Title: MicroRNA-26a inhibits TGF-β-induced extracellular matrix protein expression in podocytes by targeting CTGF and is downregulated in diabetic nephropathy.
Article Snippet: .. Transfection Differentiated podocytes were transfected with miR-26a mimics or mimic negative control (NC; 30 nmol/l), miR-26a inhibitors or inhibitor NC (30 nmol/l), and siRNA for CTGF, CTDSP2 and CTDSPL (5 nmol/l) by a pulse of electroporation with Nucleofector (Amaxa Biosystems, Cologne, Germany) using the Basic Nucleofector Kit for Primary Mammalian Epithelial cells (Amaxa Biosystems) [29] (for further details, please refer to the ESM Methods). ..

Article Title: Post-stroke hippocampal neurogenesis is impaired by microvascular dysfunction and PI3K signaling in cerebral amyloid angiopathy.
Article Snippet: ReN cells were seeded in a 6-well culture plate coated with laminin and immediately transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit.78 For gene silencing, cells were transfected with 0.5 mg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer SDF1 (CXCL12)-siRNAs 3 unique 27mer PI3KC2A-siRNAs, 3 unique 27mer CREB3L2-siRNAs, or trilencer-27 universal scrambled (SCR) silencer siRNA duplex as a negative control. .. ReN cells were seeded in a 6-well culture plate coated with laminin and immediately transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit.78 For gene silencing, cells were transfected with 0.5 mg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer SDF1 (CXCL12)-siRNAs 3 unique 27mer PI3KC2A-siRNAs, 3 unique 27mer CREB3L2-siRNAs, or trilencer-27 universal scrambled (SCR) silencer siRNA duplex as a negative control. ..

Electroporation:

Article Title: MicroRNA-26a inhibits TGF-β-induced extracellular matrix protein expression in podocytes by targeting CTGF and is downregulated in diabetic nephropathy.
Article Snippet: .. Transfection Differentiated podocytes were transfected with miR-26a mimics or mimic negative control (NC; 30 nmol/l), miR-26a inhibitors or inhibitor NC (30 nmol/l), and siRNA for CTGF, CTDSP2 and CTDSPL (5 nmol/l) by a pulse of electroporation with Nucleofector (Amaxa Biosystems, Cologne, Germany) using the Basic Nucleofector Kit for Primary Mammalian Epithelial cells (Amaxa Biosystems) [29] (for further details, please refer to the ESM Methods). ..

Article Title: Generation and characterization of giant panda induced pluripotent stem cells
Article Snippet: GPFs at early passages were isolated through trypsin treatment (0.25% trypsin/0.5 mM EDTA, Gibco) and subjected to electroporation with specified episomal plasmids. .. This process used the Amaxa Basic Nucleofector Kit for primary fibroblasts cells, program T-020 (Lonza), or cells could be resuspended in phosphate-buffered saline (PBS) solution and electroporated using Gene Pulser MXcell Electroporation System (Bio-Rad). .. The electroporated GPFs were seeded onto Matrigel (BD) precoated P6 wells.

Saline:

Article Title: Generation and characterization of giant panda induced pluripotent stem cells
Article Snippet: GPFs at early passages were isolated through trypsin treatment (0.25% trypsin/0.5 mM EDTA, Gibco) and subjected to electroporation with specified episomal plasmids. .. This process used the Amaxa Basic Nucleofector Kit for primary fibroblasts cells, program T-020 (Lonza), or cells could be resuspended in phosphate-buffered saline (PBS) solution and electroporated using Gene Pulser MXcell Electroporation System (Bio-Rad). .. The electroporated GPFs were seeded onto Matrigel (BD) precoated P6 wells.

Small Interfering RNA:

Article Title: Post-stroke hippocampal neurogenesis is impaired by microvascular dysfunction and PI3K signaling in cerebral amyloid angiopathy.
Article Snippet: ReN cells were seeded in a 6-well culture plate coated with laminin and immediately transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit.78 For gene silencing, cells were transfected with 0.5 mg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer SDF1 (CXCL12)-siRNAs 3 unique 27mer PI3KC2A-siRNAs, 3 unique 27mer CREB3L2-siRNAs, or trilencer-27 universal scrambled (SCR) silencer siRNA duplex as a negative control. .. ReN cells were seeded in a 6-well culture plate coated with laminin and immediately transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit.78 For gene silencing, cells were transfected with 0.5 mg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer SDF1 (CXCL12)-siRNAs 3 unique 27mer PI3KC2A-siRNAs, 3 unique 27mer CREB3L2-siRNAs, or trilencer-27 universal scrambled (SCR) silencer siRNA duplex as a negative control. ..



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